Original article

BioMedicine

, 4:4

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Vascular Endothelial Growth Factor -460 C/T BstUI Gene Polymorphism is associated with Primary Open Angle Glaucoma

  • Hui-Ju LinAffiliated withDepartment of Ophthalmology, China Medical University HospitalSchool of Chinese Medicine, China Medical University
  • , Wen-Lu ChenAffiliated withDepartment of Ophthalmology, China Medical University HospitalSchool of Chinese Medicine, China Medical University
  • , Ter-Hsin ChenAffiliated withGraduate Institute of Veterinary Pathobiology, College of Veterinary Medicine, National Chung Hsing University
  • , Yung-Jen KungAffiliated withDepartment of Veterinary Medicine, National Chung Hsing University
  • , Lei WanAffiliated withSchool of Chinese Medicine, China Medical UniversityDepartment of Biotechnology, Asia UniversityDepartment of Gynecology, China Medical University Hospital Email author 

Abstract

Background

Hypoxia and nitric oxide (NO) play important roles in the onset and progression of glaucoma. Vascular endothelial growth factor (VEGF) is one of the main factors responsive to hypoxia and NO. In this study, we investigated the association between the BstUI C/T VEGF gene polymorphism and primary open angle glaucoma (POAG).

Methods

60 POAG patients and 78 healthy volunteers were enrolled in this study. The most frequently observed polymorphism in the VEGF gene is BstUI C/T, which was located 460 nucleotides upstream of the gene. The polymorphism was observed using polymerase chain reaction-based restriction analysis.

Results

Significant differences were observed in the distribution of the polymorphism between control subjects and POAG patients (p = 0.003). C/C homozygotes are absent in the control group; therefore, this genotype represents a suitable genetic maker for POAG.

Conclusions

Hypoxia and NO may be involved in the pathway whereby the VEGF-460 polymorphism regulates POAG. Furthermore, homozygous C/C VEGF genotype is a useful maker for Chinese POAG.

Background

Hypoxia and nitric oxide (NO) play important roles in the onset and progression of glaucoma. Vascular endothelial growth factor (VEGF) is one of the main factors responsive to hypoxia and NO. In this study, we investigated the association between the BstUI C/T VEGF gene polymorphism and primary open angle glaucoma (POAG).

Methods

60 POAG patients and 78 healthy volunteers were enrolled in this study. The most frequently observed polymorphism in the VEGF gene is BstUI C/T, which was located 460 nucleotides upstream of the gene. The polymorphism was observed using polymerase chain reaction-based restriction analysis.

Results

Significant differences were observed in the distribution of the polymorphism between control subjects and POAG patients (p = 0.003). C/C homozygotes are absent in the control group; therefore, this genotype represents a suitable genetic maker for POAG

Conclusions

Hypoxia and NO may be involved in the pathway whereby the VEGF-460 polymorphism regulates POAG. Furthermore, homozygous C/C VEGF genotype is a useful maker for Chinese POAG.

Keywords

Primary open angle Glaucoma Genetic polymorphism Vasogenetic endogenous Growth factor Nitric oxide Hypoxia