Original article

BioMedicine

, 5:9

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

The medical diagnostic approaches with phylogenetic analysis for rare Brucella spp. diagnosis in Taiwan

  • Ni TienAffiliated withDepartment of Laboratory Medicine, China Medical University HospitalDepartment of Medical Laboratory Science and Biotechnology, China Medical University
  • , Yun-Ju SungAffiliated withDepartment of Laboratory Medicine, China Medical University Hospital
  • , Yi-Chih ChangAffiliated withDepartment of Medical Laboratory Science and Biotechnology, China Medical University
  • , Bang-Jau YouAffiliated withDepartment of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University
  • , Michelle LiangAffiliated withDepartment of Microbiology, UCLA
  • , Yun-Ping LimAffiliated withDepartment of Pharmacy, College of Pharmacy, China Medical University
  • , Wen-Yu HoAffiliated withDepartment of Laboratory Medicine, China Medical University Beigang Hospital
  • , Hsiu-Shen LinAffiliated withDepartment of Laboratory Medicine, China Medical University Hospital
  • , Mao-Wang HoAffiliated withDepartment of Internal Medicine, School of Medicine, China Medical University
    • , Chen-Mao HoAffiliated withDepartment of Laboratory Medicine, China Medical University HospitalDepartment of Internal Medicine, School of Medicine, China Medical University
    • , Chao-Chin ChangAffiliated withGraduate Institute of Microbiology and Public Health, National Chung Hsing University
    • , Yu-Ching LanAffiliated withDepartment of Health Risk Management, China Medical University Email author 

Abstract

Brucellosis is a bacterial zoonotic disease which can be easy to misdiagnose in clinical microbiology laboratories. In the present study, we have tried to improve the current clinical method for detecting Brucella spp. and its antibiotic characteristics. Our method begins with detecting the clinical isolate through traditional biochemical methods and automatic identification systems. Then, we move on to editing the sequence for BLAST allows us to compare 16s rRNA sequences with sequences from other species, allowing the gene level to be determined. Next, the phylogenetic analysis of multiple genetic loci is able to determine the evolutionary relationships between our bacteria strain and those from other locations. Finally, an anti-microbial susceptibility test hones in on the level of antibacterial activity that the bacteria displays. Employing these four steps in concert is extremely effective in identifying rare bacteria. Thus, when attempting to determine the identity of rare bacteria such as Brucella, utilizing these four steps from our research should be highly effective and ultimately prevent further identification errors and misdiagnoses. The standards we have suggested to identify rare bacteria strains is applicable not only to Brucella, but also to other rarely encountered bacteria.

Keywords:

Brucella Traditional biochemical methods 6s rRNA Phylogenetic analysis Anti-microbial susceptibility test