Article

BioMedicine

, 5:15

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Protective effects of (-)-epigallocatechin-3-gallate against acetaminophen-induced liver injury in rats)

  • Hsien-Tsung YaoAffiliated withDepartment of Nutrition, China Medical University Email author 
  • , Yu-Chi YangAffiliated withDepartment of Nutrition, China Medical University
  • , Chen-Hui ChangAffiliated withDepartment of Nutrition, China Medical University
  • , Hui-Ting YangAffiliated withDepartment of Nutrition, China Medical University
  • , Mei-Chin YinAffiliated withDepartment of Nutrition, China Medical University

Abstract

(-)-Epigallocatechin-3-gallate (EGCG) is the most abundant catechin with various biological activities found in tea. In this study, the effects of EGCG on the metabolism and toxicity of acetaminophen in rat liver were investigated. Male Sprague-Dawley rats were fed a controlled diet without or with EGCG (0.54 %, w/w) for 1 week and were then intraperitoneally injected with acetaminophen (1 g/kg body weight) and killed after 12 h. Concentrations of acetaminophen and its conjugates in plasma and liver were then determined. The cytochrome P450 (CYP) and phase II enzymes activities were also evaluated. Rats fed the EGCG diet had lower plasma alanine aminotransferase and aspartate aminotransferase activities, as indices of hepatotoxicity, after acetaminophen treatment. Morphological damage by acetaminophen was lower in rats fed the EGCG diet. In addition, EGCG significantly reduced hepatic activities of midazolam 1-hydroxylation (CYP3A), nitrophenol 6-hydroxylase (CYP2E1), UDP-glucurosyltransferase, and sulfotransferase. Finally, EGCG feeding reduced acetaminophen-glucuronate and acetaminophen-glutathione contents in plasma and liver. These results indicate that EGCG feeding may reduce the metabolism and toxicity of acetaminophen in rats.

Keywords:

(-)-Epigallocatechin-3 gallate; Acetaminophen; Cytochrome P450; Hepatotoxicity; Rats