Original research

Biomedical Research and Therapy

, 1:10

First online:

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Human umbilical cord blood derived mesenchymal stem cells were differentiated into pancreatic endocrine cell by Pdx-1 electrotransfer

  • Phuoc Thi-My NguyenAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University Email author 
  • , Anh Thai-Quynh NguyenAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Nhung Thi NguyenAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Nguyet Thi-Minh NguyenAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Thu Thi DuongAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Nhung Hai TruongAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Ngoc Kim PhanAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University

Abstract

Diabetes mellitus type 1 is an autoimmune disease with high incidence in adolescents and young adults. A seductive approach overcomes normally obstacles treatment is cell-replacement therapy to endogenous insulin production. At the present, to get enough pancreatic endocrine cells (PECs) in cell transplantation, differentiation of mesenchymal stem cells (MSCs) into IPCs is an interesting and promising strategy. This study aimed to orient umbilical cord blood-derived MSCs (UCB-MSCs) to PECs by Pdx-1 electrotransfer. UCB-MSCs were isolated from human umbilical cord blood according to published protocol. Pdx-1 was isolated and cloned into a plasmid vector. Optimal voltage of an electrotransfer was investigated to improve the cell viability and gene transfection efficacy. The results showed that 200V of the electrotransfer significantly increased in the efficiency of electrotransfer and survival cells compared with other high voltages (350V and 550V). Pdx-1 successfully transfected UCB-MSCs over-expressed pancreatic related genes as Ngn3, Nkx6.1. These results suggested that Pdx-1 transfected UCB-MSCs were successfully oriented PECs. Different to lentiviral vectors, electrotransfer is a safer method to transfer Pdx-1 to UCB-MSCs and a useful tool in translational research.

Keywords

Diabetes Electrotransfer Endocrine pancreatic Mesenchymal stem cell Pdx-1 Umbilical cord blood