Original Research

Biomedical Research and Therapy

, 1:21

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

  • Phuc Van PhamAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National UniversityLaboratory of Animal Physiology and Biotechnology, Biology Faculty, University of Science, Vietnam National University Email author 
  • , Ngoc Bich VuAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Nhan Lu-Chinh PhanAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Dung Minh LeAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Nhat Chau TruongAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National University
  • , Nhung Hai TruongAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National UniversityLaboratory of Animal Physiology and Biotechnology, Biology Faculty, University of Science, Vietnam National University
  • , Khanh Hong-Thien BuiAffiliated withHo Chi Minh University of Medicine and Pharmacy, University of Medical Center
  • , Ngoc Kim PhanAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Vietnam National UniversityLaboratory of Animal Physiology and Biotechnology, Biology Faculty, University of Science, Vietnam National University

Abstract

Adipose-derived stem cells (ADSCs) are excellent for regenerative medicine. Like mesenchymal stem cells, ADSCs possess multi-potent differentiation capacity that enables them to differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into other cells. ADSC transplantation has gained attention in recent years, especially in vitro expanded ADSC transplantation. This study aimed to provide a new method to in vitro primarily culture and secondary culture of ADSCs that were compliant with good manufacturing practice for clinical applications. Stromal vascular fraction (SVF) was extracted from adipose tissue by commercial kits. SVF was expanded in vitro in medium with non-allogeneic supplements. Cultured ADSCs maintained immune-phenotype, karyotype, and differentiation potential after ten passages. Moreover, ADSCs at 15th passage could not form tumors in NOD/SCID mice. This research produced a suitable protocol for clinical applications of expanded ADSCs.

Keywords

Adipose derived stem cells Stem cell transplantation ADSC transplantation ADSC application Stem cell culture