Original research

Biomedical Research and Therapy

, 2:10

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Therapeutic use of fisetin, curcumin, and mesoporous carbon nanoparticle loaded fisetin in bleomycin-induced idiopathic pulmonary fibrosis

  • Ena Ray BanerjeeAffiliated withImmunology and Regenerative Medicine Research Laboratory, University of Calcutta Email author 
  • , Srabani KarAffiliated withImmunology and Regenerative Medicine Research Laboratory, University of Calcutta
  • , Surajlata KonsamAffiliated withImmunology and Regenerative Medicine Research Laboratory, University of Calcutta
  • , Garima HoreAffiliated withImmunology and Regenerative Medicine Research Laboratory, University of Calcutta
  • , Shinjini MitraAffiliated withImmunology and Regenerative Medicine Research Laboratory, University of Calcutta
  • , Silpak BiswasAffiliated withImmunology and Regenerative Medicine Research Laboratory, University of Calcutta
  • , Aryabaran SinhaAffiliated withCentre for Advanced Materials, Indian Association for the Cultivation of Science
  • , Nikhil Ranjan JanaAffiliated withCentre for Advanced Materials, Indian Association for the Cultivation of Science

Abstract

Idiopathic pulmonary fibrosis (IPF) is a devastating disease of unknown etiology, for which there is no curative pharmacological therapy. Bleomycin, an anti-neoplastic agent that causes lung fibrosis in human patients has been used extensively in rodent models to mimic IPF. The conventional therapy has been steroids and immunosuppressive agents. But only a minority of patients responds to such a therapy. IPF is a progressive, ultimately fatal disorder for which substantive medical therapy is desperately needed. Fisetin is a flavonol which inhibits the activity of several pro-inflammatory cytokines. The polyphenol curcumin is used to treat inflammatory diseases, abdominal disorders, and a variety of other ailments. The aim of this study was to evaluate the beneficial effect of fistine, curcumin and mesoporous carbaon nanoparticle (MCN) loaded fisetin as an anti-inflammatory agents against bleomycin-induced changes in mice with IPF. In our study, flavonoids showed their anti fibrotic action. The inflammatory cell count was greatly increased for bleo treated individuals and effectiveness of fisein was increased after addition of MCN particles with it, curcumin also showed anti- inflammatory effects. In another experiment, bleomycin effectively inhibits the cellular recruitment to the spleen and treatment with fisetin, and curcumin increases the cellular recruitment in spleen. Colony count was also increased in MCN+fisetin treated groups, and it was statistically significant. We also observed the increased level of cytokines with fisetin treatment, with curcumin treatment and with MCN +fisetin treatment as compared to the bleo treated sample. In conclusion, the present research suggests that fisetin and curcumin and MCN loaded fisetin may be a promising therapeutic agent for bleomycin-induced changes in mice with IPF. This will open up new perspectives for a potential role of these drugs as a molecular target in Idiopathic pulmonary fibrosis.

Keywords

Inflammation anti-oxidant phenolic compounds scavenger activities reactive oxygen nitrogen intermediates nanoparticles fisetin curcumin functional food