Original research

Biomedical Research and Therapy

, 2:17

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Ex-vivo cytotoxic, antibacterial and DPPH free radical scavenging assay with ethanolic leaf extract of Glycosmis pentaphylla to justify its traditional use

  • Prawej AnsariAffiliated withDepartment of Pharmacy, International Islamic University Chittagong Email author 
  • , AKM Riasat Ul IslamAffiliated withDepartment of Pharmacy, International Islamic University Chittagong
  • , AnaytullaAffiliated withDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University
  • , Mahmuda SultanaAffiliated withState University of Bangladesh
  • , Mohammad Nazmul AlamAffiliated withDepartment of Pharmacy, International Islamic University Chittagong
  • , Mohammad MustakimAffiliated withDepartment of Pharmacy, International Islamic University Chittagong
  • , Md. Nasir UddinAffiliated withNorthern University



Glycosmis pentaphylla belongs to the family Rutaceae. It is a shrub and locally common in the treatment of hepatic impairment. We have designed this study to provide a scientific basis with the traditional use of leaf of G. pentaphylla in the treatment of hepatitis.


The well-established DPPH free radical scavenging activity was tested for antioxidant property evaluation. On the other hand, disk diffusion and brine shrimp method was respectivelyused to determine antibacterial and cytotoxic activity.

Results & Discussion:

In the evaluation of antioxidant property IC50 found 204.91 ± 2.223μg/ml, in cytotoxicity testing, it is found that the plant part shows 30.49 ± 1.976μg/ml of LC50. The ethanolic extract of G. pentaphylla leaves also have efficiency in bacterial growth inhibition; this extract is effective against for both gram, negative and positive. The zone of inhibition at 500 μg/ml dose in E. coli and C. albican culture was 18 mm and 15 mm, respectively. In thin layer chromatography analysis, we found presence of couple of non-polar and polar component, presence of three non-chromatophoric component are also evident.


Appropriate isolation and identification of mechanism is suggested in further study.


Antimicrobial Antioxidant Cytotoxic G. pentaphylla TLC