Original Research

Biomedical Research and Therapy

, 2:23

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

In vitro anti-oxidant and in vivo anti-inflammatory activity determination of the methanolic leaves extract of Millettiapachycarpa

  • Shofiul AzamAffiliated withDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmaceutical Sciences, North South University Email author 
  • , Prawej AnsariAffiliated withDepartment of Pharmaceutical Sciences, North South University
  • , Mohammad Mamun Ur RashidAffiliated withDepartment of Pharmaceutical Sciences, North South University
  • , Mohammad Nazmul AlamAffiliated withDepartment of Pharmaceutical Sciences, North South University
  • , Ismail Hussein AhmedAffiliated withDepartment of Pharmaceutical Sciences, North South University
  • , Mubarik Yusuf IbarahimAffiliated withDepartment of Pharmaceutical Sciences, North South University
  • , Shah Mohammed ShafiAffiliated withDepartment of Pharmaceutical Sciences, North South University
  • , Sadequr RahmanAffiliated withDepartment of Pharmacy, International Islamic University Chittagong
  • , Altaf HossenAffiliated withDepartment of Pharmacy, East West University

Abstract

Objective:Millettiapachycarpais a member of the Fabacea family and our object has been set to examine the anti-oxidant and anti-inflammatory endeavor of this plant. This plant has been reported as potential anti-inflammatory agent previously.Materials and Method: DPPH free radical scavenging and reducing power assay was carried out to assess the electron donating capability of the methanolic crude extract of the specimen. A quantitative analysis has been made to measure phenolic and flavonoid content. The anti-inflammatory potentiality of methanolic extract of M. pachycarpa has been determined via carrageenan-induced paw edema assay in rats.Results: The IC50 value estimated was, 196.47 ± 2.061 μg/ml, which is a very much promising result. From quantitative assessment, sample extract reported very good number of phenolic and flavonoid measurement, 41.23 ± 1.527 mg equivalent gallic acid per gram and 110.33 ± 2.255 mg equivalent rutin per gram, respectively. The studied specimen showed expected dose dependent result in anti-inflammatory assay. At the dose of 300 mg/kg the extract showed maximum inhibitory effect (61.36% at 2 hour; P <0.001) on paw swelling due to Carrageenan injection.Conclusion: This finding have proved that methanolic extract of M. pachycarpapossess potent anti-inflammatory activity possibly due to presence of good quantity flavonoid and phenolic content, also have good anti-oxidant property.

Keywords

anti-inflammatory anti-oxidant Carrageenan DPPH scavenging flavonoid M. pachycarpa