Original Research

Biomedical Research and Therapy

, 2:28

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Production of dendritic cells and cytokine-induced killer cells from banked umbilical cord blood samples

  • Phuc Van PhamAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Viet Nam National University Email author 
  • , Binh Thanh VuAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Viet Nam National University
  • , Viet Quoc PhamAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Viet Nam National University
  • , Phong Minh LeAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Viet Nam National University
  • , Hanh Thi LeAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Viet Nam National University
  • , Ngoc Kim PhanAffiliated withLaboratory of Stem Cell Research and Application, University of Science, Viet Nam National University

Abstract

Umbilical cord blood (UCB) is considered to be a source of hematopoietic stem cells (HSCs). All UCB banks have recently become interested in the isolation and storage of HSCs for the treatment of hematological diseases. However, UCB was also recently confirmed as a source of immune cells for immunotherapy such as dendritic cells (DCs) and cytokine-induced killer cells (CIKs). This study aimed to exploit this source of immune cells in banked UCB samples. After collection of UCB samples, mononuclear cells (MNCs) containing stem cells, progenitor cells, and mature cells were isolated by Ficoll-Hypaque-based centrifugation. The MNCs were subjected to freezing and thawing according to a previously published protocol. The banked MNCs were used to produce DCs and CIKs. To produce DCs, MNCs were induced in RPMI 1640 medium supplemented with GM-CSF (50 ng/ml) and IL-4 (40 ng/ml) for 14 days. To produce CIKs, MNCs were induced in RPMI 1640 medium supplemented an anti-CD3 monoclonal antibody, IL-3, and GMC-SF for 21–28 days. Both DCs and CIKs were evaluated for their phenotypes and functions according to previously published protocols. The results showed that banked UCB samples can be successfully used to produce functional DCs and CIKs. These samples are valuable sources of immune cells for immunotherapy. The present results suggest that banked UCB samples are useful not only for stem cell isolation, but also for immune cell production.

Keywords

Banked UCB Stem cells Immune cells Dendritic cells Cytokine-induced killer cells Immunotherapy