Original Research

Biomedical Research and Therapy

, 2:31

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Investigation of invivo anti-diarrheal and invitro anti-helminthic properties of methanolic leaves extract of Dalbergiastipulacea roxb

  • Anayt UllaAffiliated withDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University
  • , A.T.M. Mostafa KamalAffiliated withDepartment of Pharmacy, International Islamic University Chittagong
  • , Prawej AnsariAffiliated withDepartment of Pharmacy, International Islamic University ChittagongDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University Email author 
  • , Nasrin SultanaAffiliated withDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University
  • , Md. Hossan SakibAffiliated withDepartment of Pharmacy, University of Science and Technology (USTC)
  • , Md. RaihanAffiliated withDepartment of Pharmaceutical Sciences, School of Health and Life Sciences, North South University
  • , Mohammad Mosharraf HossainAffiliated withDepartment of Pharmacy, University of Science and Technology (USTC)

Abstract

Aim:

This study is designed to identify and evaluate the pharmacological property of leaf extract of D. stipulacea, commonly known as Horoiludi in Bangladesh.

Materials and Methods:

The antidiarrheal activity was estimated using Galvez et al. method with a simple modification. The testing extract was compared to standard Loperamide (5 mg/kg, p.o.), while the extract had two different concentrations 200 mg/kg and 400 mg/kg. On the other hand,antihelminthic property of this plant was investigated using Panagrellusredivivus worms.

Result:

At maximum concentration (400 mg/kg) it inhibited 42.22% and loperamide was found to inhibit 62.23%. The one-way ANOVA for this testing result in P < 0.0001, which means that the investigation results have significant importance in consideration of statistical analysis. The antihelminthic testing also results in a dose dependent manner. At highest dose (2.5 mg) the extract paralyzed experimental worm in 5.5 ± 1.323 min and it died in 23.93 ± 1.901 min.

Conclusion:

Based on the data analysis obtained from the study, it can be concluded that the plant part has good anti-diarrheal activity as well as antihelminthic property. We need to identify its actual molecule through further processing.

Keywords

Anti-diarrheal antihelminthic D. stipulacea Panagrellusredivivus