Original Research

Biomedical Research and Therapy

, 3:33

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Extenuating the role of Ficus virens Ait and its novel bioactive compound on antioxidant defense system and oxidative damage in cigarette smoke exposed rats

  • Danish IqbalAffiliated withClinical Biochemistry and Natural Product Research Laboratory, Department of Biosciences, Integral University LucknowDepartment of Medical Laboratory Sciences, College of Applied medical Sciences, Majmaah University
  • , M. Salman KhanAffiliated withClinical Biochemistry and Natural Product Research Laboratory, Department of Biosciences, Integral University Lucknow Email author 
  • , Amir KhanAffiliated withDepartment of Maxillofacial Surgery (Biochemistry), College of Dentistry, Taif University
  • , Saheem AhmadAffiliated withClinical Biochemistry and Natural Product Research Laboratory, Department of Biosciences, Integral University Lucknow

Abstract

Introduction:

Production of free radicals is associated with cigarette smoke (CS) which in turn generates oxidative stress, could be responsible for alterations in the activities of enzymatic and non-enzymatic antioxidants that links with atherosclerosis.

Methods:

Therefore, the putative preventive effects of F. virens extract and its bioactive compound (F18), n-Octadecanyl-O-α-D-glucopyranosyl(6’→1’’)-O-α-D-glucopyranoside were investigated on overall enzyme and non-enzymatic defense system and in oxidative stress CS-exposed rats.

Results:

The enzymatic activities of hepatic and lung CAT, SOD, Gred and GST in CS exposed rats were significantly decreased, while Gpx activity in CS exposed rats was increased. Similarly, hepatic and lung GSH content was reduced when compared to value of normal control group. Simultaneous administration of FVBM extract (50 and 100 mg/rat) and F18 bioactive compound (1 mg/rat) significantly increases hepatic and lung CAT, SOD, Gred and GST activity as well GSH concentration coupled with decrease in Gpx level in CS-exposed stress rats. Moreover, our histological observations concludes the pulmonary congestion, thickening of interalveolar septa and foci of collapsed alveoli with subsequent dilation of the adjoining alveolar spaces as well as development of large irregular spaces in rats lung exposed to cigarette smoke. Similarly, the liver also showed morphological alterations with congestion in central vein, portal inflammation and necrosis in CS-exposed rats. These morphological changes reversed significantly after treatment with FVBM extract and F18 compound.

Conclusion:

Thus biochemical and histopathological studies suggested that, FVBM extract and F18 showed its protective nature against CS-exposed rats.

Keywords:

Cigarette smoke Oxidative stress Antioxidant defense system Histopathology Ficus virens