Article

GSTF Journal of Psychology (JPsych)

, 1:9

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Ethanol extracts of centella asiatica leaf improves memory performance in rats after chronic stress via reducing nitric oxide and increasing Brain-Derived Neurotrophic Factor (BDNF) Concentration

  • Dwi Cahyani Ratna SariAffiliated withDepartment of Anatomy, Embryology, and Anthropology Faculty of Medicine, Universitas Gadjah Mada Email author 
  • , Soedjono AswinAffiliated withDepartment of Anatomy, Embryology, and Anthropology Faculty of Medicine, Universitas Gadjah Mada
  • , Rina SusilowatiAffiliated withDepartment of Histology and Cell Biology, Faculty of Medicine, Universitas Gadjah Mada
  • , Mawaddah Ar-RochmahAffiliated withDepartment of Anatomy, Embryology, and Anthropology Faculty of Medicine, Universitas Gadjah Mada
  • , Djoko PrakosaAffiliated withDepartment of Anatomy, Embryology, and Anthropology Faculty of Medicine, Universitas Gadjah Mada
  • , Mansyur RomiAffiliated withDepartment of Anatomy, Embryology, and Anthropology Faculty of Medicine, Universitas Gadjah Mada
  • , Untung TranggonoAffiliated withDepartment of Surgery, Faculty of Medicine, Universitas Gadjah Mada
  • , Nur ArfianAffiliated withDepartment of Anatomy, Embryology, and Anthropology Faculty of Medicine, Universitas Gadjah Mada

Abstract

Abstract- Background: Centella asiatica is herbaceous plant that has medicinal value to improve learning and memory. Brain- derived neurotrophic factor (BDNF) has a significant role in memory formation process, while oxidative stress causes memory impairment. Objective: This study aimed to investigate the effects of ethanol extracts of Centella asiatica leaf on memory performance and serum BDNF (neurotrophins) and Nitric Oxide(NO) concentration of rats following chronic electrical stress. Materials and Methods : Twenty male rats (Sprague Dawley) were divided into four groups: control/aquadest group and groups treated with three different doses (mg/kg) of Centella asiatica :150 (CeA150), 300 (CeA300) and 600 (CeA600). Memory performance was tested using probe test in Morris Water Maze before and after oral administration of ethanol extracts of Centella asiatica leaf followed by electrical stress for 28 days. At the last day of memory exercise following chronic stress, blood sample was taken from periorbital veins of all rats. Concentration of serum BDNF and total NO was assessed using ELISA. Data were analyzed using one way ANOVA. Results: Treatment groups had higher percentage in probe test water maze and serum BDNF concentration after chronic stress. Mean time percentage (%) of probe test in water maze performance for control group, CeA150, CeA300, and CeA600 were 53.33±2.43, 55.78±4.16, 63.33±4.07, and 73.55±3.29 (p<0.05). Mean concentration of serum BDNF (ng/ml) for control group, CeA150, CeA300, and CeA600 were 1.52±0.89, 1.49±0.20, 1.73±0.15, and 2.16±0.20 (p<0.05 vs control group). Control group had higher serum NO concentration compared to all treatments groups. Mean concentration of serum NO (μmol/L) for control group, CeA150, CeA300, and CeA600 were 5.94±0.52, 3.98±0.35, 3.06±0.33, and 2.82±0.21 (p<0.05). Conclusion: Ethanol extracts of Centella asiatica leaf increases memory performance and serum BDNF concentration, and also decreases Nitric Oxide levels in rats after chronic stress.

Keywords

learning and memory brain-derived neurotrophic factor; nitric oxide Centella asiatica