Article

GSTF Journal of Chemical Sciences (JChem)

, 1:1

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Analysis of Salicylic Acid in Tobacco Leaves Using Capillary Zone Electrophoresis with UV Detection

  • Wei-Ting ChenAffiliated withDepartment of Chemistry, National Changhua University of Education
  • , Chiung-I WangAffiliated withDepartment of Chemistry, National Changhua University of Education
  • , Shih-Feng FuAffiliated withDepartment of Biology, National Changhua University of Education
  • , Yang-Wei LinAffiliated withDepartment of Chemistry, National Changhua University of Education

Abstract

A simple and inexpensive capillary zone electrophoresis (CZE) with UV detection method was used to determine salicylic acid (SA) and six structural analogs (benzoic acid, 3-hydroxybenzoic acid, 4-hydroxybenzoic acid, acetylsalicylic acid, methyl salicylate, and jasmonic acid). All of the compounds were successfully separated within a migration period of 6 min, with a high number of theoretical plates (>37,000, calculated using the width at the base of the SA peak), in 20.0 mmol L-1 Na2HPO4/H3BO3 buffer (pH 9.0) containing 2.0% methanol and 2.0% acetonitrile. The regression line for SA was linear over the range 0.5–200 µmol L-1. Both the intra- and inter-day precisions of the migration time (relative standard deviations, RSDs, 0.6% and 4.7%, respectively) and peak area (RSDs 0.9% and 7.3%, respectively) were acceptable. The proposed method was used to determine the SA concentrations in tobacco leaves ( Nicotiana tabacum L.) from the Xanthi-nc ( NN genotype) and the Nt-NahG mutant strains that had been irradiated with UV (254 nm) for 20 min and the ones that had not been irradiated. Rapid separation (<6 min), good reproducibilities (RSDs < 11.9%), and good recoveries (94.7–118%) were obtained in the tobacco leaf analyses.

Keywords

Tobacco leaves salicylic acid capillary zone electrophoresis UV detection