Article

GSTF Journal of Chemical Sciences (JChem)

, 1:2

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Application of Adsorption Chromatography in the Papain Processing

Effect on Recovery of Papain from Aqueous Solutions
  • He YuhaiAffiliated withFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang
  • , Syed mohd saufi Bin Tuan ChikAffiliated withFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang
  • , Chong Fui ChinAffiliated withFaculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang Email author 

Abstract

A direct recovery of papain enzyme from unclarified Carica papaya juice was developed successfully using a reversed phase expanded bed adsorption chromatography (RP-EBAC). The dynamic binding capacity for the RP-EBAC at 10% breakthrough of 810.52 μg papain/g adsorbent was achieved at a linear flow velocity of 936 cm/h, bed expansion of two and feedstock viscosity of 2.68 mPa·s. Two-step elution was employed to enhance the purity of papain in RP-EBAC which gives high papain purity of 74.98% and high purification factor of 7.04. This work shows a great potential of using RP-EBAC with elution optimization step to purify papain from unclarified Carica papaya juice.

Key words

Papain Chromatography RP-EBAC Purification