Article

GSTF Journal of Veterinary Science (JVet)

, 3:1

First online:

Open Access This content is freely available online to anyone, anywhere at any time.

Exploring the transcriptomic data of the Australian paralysis tick, Ixodes holocyclus

  • CT OngAffiliated withQueensland Alliance for Agriculture & Food Innovation, The University of Queensland
  • , M Rodriguez-ValleAffiliated withQueensland Alliance for Agriculture & Food Innovation, The University of Queensland Email author 
  • , PM MoolhuijzenAffiliated withCentre for Comparative Genomics, Murdoch University
  • , RA BarreroAffiliated withCentre for Comparative Genomics, Murdoch University
  • , A HunterAffiliated withCentre for Comparative Genomics, Murdoch University
  • , T SzaboAffiliated withCentre for Comparative Genomics, Murdoch University
  • , MI BellgardAffiliated withCentre for Comparative Genomics, Murdoch University
  • , AE Lew-TaborAffiliated withQueensland Alliance for Agriculture & Food Innovation, The University of QueenslandCentre for Comparative Genomics, Murdoch University Email author 

Abstract

Ixodes holocyclus is the paralysis tick commonly found in Australia. I. holocyclus does not cause paralysis in the primary host – bandicoots, but markedly affects secondary hosts such as companion animals, livestock and humans. Holocyclotoxins are the neurotoxin molecules in I. holocyclus responsible for paralysis symptoms. There is a limited understanding of holocyclotoxins due to the difficulties in purifying and expressing these toxins in vitro. Next-generation sequencing technologies were utilised for the first time to generate transcriptome data from two cDNA samples –salivary glands samples collected from female adult ticks engorged on paralysed companion animals and on bandicoots. Contig-encoded proteins in each library were annotated according to their best BLAST match against several databases and functionally assigned into six protein categories: housekeeping, transposable elements, pathogen-related, hypothetical, secreted and novel. The “secreted protein” category is comprised of ten protein families: enzymes, protease inhibitors, antigens, mucins, immunity-related, lipocalins, glycine-rich, putative secreted, salivary and toxin-like. Comparisons of contig representation between the two libraries reveal the differential expression of tick proteins collected from different hosts. This study provides a preliminary description of the I. holocyclus tick salivary gland transcriptome.

Keywords

Salivary Gland Salivary Protein Glycine Rich Protein Tick Saliva Kunitz Domain